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KMID : 0381120220440080913
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Genes and Genomics
2022 Volume.44 No. 8 p.913 ~ p.922
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ICT1 deficiency leads to reduced oxygen resistance due to the cell wall damage in S. cerevisiae
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Zhu Hong
Wang Mengfei
Zhou Hua
Cai Heng
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Abstract
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Background: Disruption of ICT1 has been known to cause a significant deficiency in the phospholipid composition which is necessary for cell stress adaptation. However, the effects of ICT1 deletion on antioxidant research are not clear.
Objective: Construct a knockout strain to investigate the efficacy of ICT1 on antioxidant activity.
Methods: The antioxidant-related genes and phospholipid-related genes were determined by RT-PCR, the cell wall shape was observed by TME, and CWI pathway phosphorylation experiments were also analyzed by HPLC.
Results: The expression of antioxidant related genes and phospholipid-related genes has a slight reduction compared to the wild type. The cell wall was observed impaired with apparent CWI pathway phosphorylation weakening in the mutant.
Conclusion: These findings indicate the role of ICT1 on antioxidant activity because it not only directly affects phospholipid composition but also further leads to the activation of CWI.
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KEYWORD
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ICT1, Oxidative stress, Plasma membrane, CWI, Saccharomyces cerevisiae
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